High Performance Liquid Chromatography (HPLC) operates by pumping a liquid sample through a chromatographic column packed with a stationary phase, typically consisting of small porous particles. The sample components are separated based on differences in their affinity for the stationary phase and the mobile phase, which is typically a liquid solvent or a mixture of solvents. HPLC offers high resolution, sensitivity, and versatility, making it suitable for analyzing a wide range of compounds, including small molecules, biomolecules, pharmaceuticals, and natural products. Different modes of HPLC, such as reverse-phase, normal-phase, ion-exchange, and size-exclusion chromatography, allow for the separation of diverse analytes with varying polarities, charges, and sizes. HPLC can be coupled with various detectors, including UV-Vis, fluorescence, mass spectrometry, and refractive index detectors, to enable detection and quantification of separated analytes. HPLC is widely used in pharmaceuticals, environmental analysis, food and beverage, clinical diagnostics, and research laboratories for quality control, process monitoring, and research purposes. Continuous advancements in column technology, instrumentation, and data analysis software improve the efficiency, sensitivity, and speed of HPLC analysis, further expanding its applications in diverse scientific disciplines.
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Thomas J Webster, Brown University, United States
Title : Synthesis of chitosan composite of metal organic framework for the adsorption of dyes, kinetic and thermodynamic approach
Tooba Saeed, University of Peshawar, Pakistan
Title : Synthesis, ADMET, PASS, molecular docking, and dynamics simulation investigation of novel octanoyl glucoopyranosides & valeroyl ribofuranoside esters.
Hasinul Babu, University of Chittagong, Bangladesh
Title : Expanding and improve the 2D periodic law of Менделееь elements, and construct the "3D periodic law of elements"
Zhongsheng Lee, Zhengzhou Commercial Technician College, China
Title : Advances in Plasma-Based Radioactive Waste Treatment
Hossam A Gabbar, Ontario Tech University, Canada
Title : Nature meets innovation: Green synthesis of nanoparticles using plant extracts and ionic liquids for a sustainable future
Azeez A Barzinjy, Soran University, Iraq