Title : Volatile chiral compound stabilization in camel hump fat lipid matrix for skincare innovation
Abstract:
Volatile chiral compounds are attracting increasing interest due to their stereoselective anti- aging, antioxidant, and anti-inflammatory actions, which make them valuable active ingredients in cosmetic and therapeutic formulations. However, their high volatility and instability during conventional extraction often lead to low yields and loss of chiral purity. Therefore, the development of sustainable methods that preserve their structural and functional integrity is essential. Ammodaucus leucotrichus is a notable natural source of bioactive chiral monoterpenoids, including representatives such as (S)-perillaldehyde. In this work, Microwave-Assisted Extraction (MAE) was evaluated as a green and efficient alternative to conventional hydrodistillation (HD) and steam distillation (SD). A Box–Behnken Design (BBD) was applied to optimize extraction parameters, including microwave power, liquid-to- solid ratio, and extraction time. Chemical characterization via GC–MS confirmed improved preservation of volatile chiral constituents using MAE compared to HD and SD. Furthermore, biological evaluation revealed enhanced antioxidant activity and remarkable anti-aging enzyme inhibition, including tyrosinase (95.23%), hyaluronidase (89.12%), collagenase (87.79%), and elastase (89.12%). These findings indicate that MAE not only improves extraction efficiency but also contributes to the stabilization and functional enhancement of volatile chiral bioactives. This reinforces their potential as next-generation multifunctional ingredients for dermo-cosmetic and pharmaceutical applications.
Keywords: Volatile chiral compounds; Ammodaucus leucotrichus; Microwave-Assisted Extraction; Stereochemical stability; Anti-aging enzymes; Dermo-cosmetic applications.
