Title : Testing the association between the UCP2 gene RS659366 with metabolic syndrome in patients with obesity from county emergency hospital Giurgiu Romania
Abstract:
Introduction. UCP2 is a protein that in humans is encoded by the UCP2 gene. The UCP2 gene is located genomically at 11q13.4 and plays a role in thermogenesis, obesity, diabetes mellitus and atherosclerosis.UCP2 has been associated with an increased risk of obesity in middle-aged individuals, and genetic variation in the UCP2 gene located at locus 4, defines the BMI polymorphism of the UCP2 gene, G/A (rs659366). The G/G and G/A genotypes have been associated with obesity. The -866G/G genotype has been associated with an increased risk of T2DM. UCP2 is a candidate gene for the development of DMT2. UCP2 plays a role in the metabolic syndrome, by downregulating insulin secretion and in the development of DMT2. The aim of the study is to test the association between the UCP2 rs659366 gene with MS in patients with obesity based on clinical data, laboratory investigations and genetic methods. Material and method. The study was conducted on 75 subjects from the Giurgiu County Emergency Hospital, 55 with obesity (OB) and 20 controls, based on clinical data: BMI, HTA, sex and age, biochemical laboratory investigations: glucose, triglycerides, cholesterol, HDL, LDL, uric acid, Magnesium, Hba1c performed on the BS300 device and hematological BS3000 Mindray for HLG: WBC, RBC, HGB, and HCT. The polymorphisms of the UCP2 rs659366 gene were sequenced by the RFLP-PCR method and the Advenced NGx test. The results were processed by the Graph Pad Prism 7.0.3, MDR 3.0.2 and Matlab R2009b programs. Results. It results that the UCP2 rs659366 polymorphisms have predominantly 20 homozygous and 9 heterozygous genotypes that showed increased values of glucose concentrations and HbA1c are associated with cardiovascular diseases, diabetes and obesity, which may increase the risk of MS. It results that the UCP2 gene with UCP2 rs659366 (OR>1 and p<0.0001, OR=2), presents an increased risk of developing MS. Conclusions. The UCP2 gene is associated with metabolic syndrome.
