Title : Optimization and validation of HPLC-DAD method for the quantification of curcuminoids in turmeric (Curcuma Longa)
Curcumin and its derivatives named curcuminoids are a naturally occurring polyphenolic compounds extensively marketed as a nutraceutical. The wide therapeutic potential of curcumin is highly explored by the authors. Therefore, many inventions were made by pharma-industries for its clinical use and to bring it to the market as a drug. In this study, a simple, rapid and sensitive High-Performance Liquid chromatographic (HPLC) method for simultaneous determinations of curcumin (C), desmethoxycurcumin (DMC) and bis-desmetoxycurcumin (BDMC) was optimized and validated.
Separation of curcumin, DMC and BDMC was performed using C18 column. Six factors including the solvent type, ratio of mobile phase, flow rate of mobile phase, column temperature, enjection volüme, and wavelength were investigated . Taking into consider retention time, peak area, resolution and tailing factor, the optimum conditions were preferred: methanol as solvent, THF/Citric A(40/60) as mobile phase, rate of mobile phase= 0,5 mL/min, temperature of column = 30 oC, injection volume= 20 microliteri and wavelength=425 nm.
- This research can help listeners about method validation and optimization in HPLC analysis.
- The method is further used to determine ppm levels of curcumin derivatives and recovery was found to be 90%.
- The proposed analytical method can be used for the quantification of curcumin in medicinal plants.