Cardiovascular diseases (CVDs) continue to be one of the leading causes of death worldwide. Currently, patients with pre-existing CVDs and risk factors are more likely to experience adverse outcomes associated with Coronavirus Disease 2019 (COVID-19). In the pathophysiological process associated with CVDs, platelet activation with the key participation of mitochondria favors the development of CVDs.
Epidemiological studies have demonstrated the cardiovascular protective role of a healthy diet (fruit and vegetables). In this context, finding bioactive compounds with biological activity that help improve the nutritional status of the population constitutes a powerful tool for the prevention of CVDs. Our research has demonstrated the antiplatelet activity of fruit and vegetable extracts widely consumed in the world and Chile, among which the tomato extract was highlighted. The Maule Region has the main tomato processing industry, annually large quantities of this industrial by-product are generated, obtained from the tomato process, called tomato pomace (made up of skin, seeds, and pulp). This product is underutilized, since it is sold at a very low price or given away for animal consumption. Despite its underutilization, previous studies by our group have observed that tomato pomace has a potent antiplatelet activity. This activity from tomato pomace could be attributed to its high content of fatty acids (> 30%). The fatty acids present in tomato pomace can nitrate during digestion, these are the product of the reaction of nitrogen dioxide with unsaturated fatty acids, so it is important to better understand the mechanisms of formation of nitrated fatty acids from tomato pomace and its antiplatelet action. We perform: "Synthesis and evaluate the antiplatelet action of nitro−fatty acids from tomato pomace.”. To this end, a methodology meeting each of the specific goals used: (1) To synthesize nitro-fatty acids from tomato pomace, (2) To isolate, chemically characterize, and quantify nitro-fatty acids from tomato pomace, and (3) To evaluate the antiplatelet activity of nitro-fatty acids from tomato pomace in the absence of the cytotoxic effects. Finally, knowledge obtained on the antiplatelet activity of nitrated fatty acids from tomato pomace will be used for the further development of new and more effective agents. Considering these points, our proposal could be particularly relevant in the context of medicinal chemistry in Chile, in addition to giving added value to this currently underused natural product despite the biological potential attributed to it.