Title : Design, Synthesis and in silico Evaluation of Novel azines Schiff-Based Compounds as anticholinesterase inhibitors.
Azines, commonly known as bis Schiff bases, are a class of important pharmacophores that undergo a wide variety of chemical and pharmacological processes. Literature survey revealed that azines were found to possess antimicrobial and anticancer activities of which makes them an attractive object of organic synthesis and arouses interest in rationalizing the methods for their preparation. Although symmetrical azines are readily synthesized by the reaction of hydrazine with an excess of an aldehydes. Herein we report the preparation and the characterizations of novel symmetrical azines and explore their biological anticholinesterase activities .1 H NMR, 13CNMR, UV-Vis, FT-IR Spectroscopy and DRX were used to characterize these azines structures. In addition, Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) evaluation inhibitory of these two new azines Schiff bases were measured in vitro and were compared to those of standard drugs. . Theoretical calculations have been carried out at DFT/B3LYP level for the optimized geometries and TD-DFT methods for vibrational frequencies. Docking studies were performed to examine potential inhibitions of the designed azines against cholinesterases.